PI-Based Separation of Tissue Proteins Realized with the Use of Two-Dimensional Gel Electrophoresis or Alternatively Capillary Isoelectric Focusing
نویسندگان
چکیده
منابع مشابه
Two−dimensional separation of human plasma proteins using agarose gel isoelectric focusing followed by SDS capillary electrophoresis
Two−dimensional polyacryiamide gel electrophoresis (2−D PAGE) has been widely used for protein separation because of its high resolution; more than one thousand proteins or polypeptides can be separated according to the differences of their isoelectric point (pI) and molecular mass. However, 2−D PAGE involves many steps of manual handling, sample injection, setting gel molds in an electrophores...
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CIEF and CZE are coupled with LIF detection to create an ultrasensitive 2-D separation method for proteins. In this method, two capillaries are joined through a buffer-filled interface. Separate power supplies control the potential at the injection end of the first capillary and at the interface; the detector is held at ground potential. Proteins are labeled with the fluorogenic reagent Chromeo...
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While two-dimensional electrophoresis (2-DE) of proteins, as we know it, was first described nearly four decades ago (O’Farrell, 1975), it continues to be a critical component of comprehensive proteomics analysis today. Continuing refinements of 2-DE, particularly the development of immobilized pH gradients (Righetti, 1990), new chaotropes (Vecchio et al. 1984, Rabilloud 1998) and detergents (S...
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A novel two-dimensional (2D) separation system for proteins was reported. In the system, a piece of dialysis hollow-fiber membrane was employed as the interface for on-line combination of capillary isoelectric focusing (CIEF) and capillary non-gel sieving electrophoresis (CNGSE). The system is similar equivalent to two-dimensional polyacrylamide gel electrophoresis (2D PAGE), by transferring th...
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ژورنال
عنوان ژورنال: The Open Proteomics Journal
سال: 2012
ISSN: 1875-0397
DOI: 10.2174/1875039701205010022